Filter Media,Media Filter,Dual Media Filter,Fluval Filter Media ANPING HANKE FILTER TECHNOLOGY CO , https://www.anpinghankefilter.com
Near-infrared two-zone fluorescence technology breakthrough imaging system successfully developed
[ Instrument R & D of Instrument Network ] NIR-II (NIR-II) refers to near-infrared light with a wavelength of 1000-1700 nm. Compared with the visible light commonly used in fluorescence imaging and the near-infrared one region, the near-infrared two region has an emission wavelength Longer, can significantly inhibit the scattering of light when penetrating biological tissues and the effect of biological tissue autofluorescence, making detection depth deeper, spatial resolution and signal-to-noise ratio higher. Therefore, the near-infrared two-zone fluorescence imaging technology has received extensive attention in recent years and has been rapidly developed.
The research of near-infrared two-zone fluorescence imaging technology mainly focuses on the development of fluorescent dyes. For example, the research team of Wuhan University has constructed the near-infrared two-zone organic small molecule dye HLZ-BTED with the aggregation-induced luminescence effect (AIE), and the research of Southern University of Science and Technology The team applied high-purity dye-antibody fluorescent probes to three-dimensional staining imaging studies of biological tissues. However, there are few studies on the optical imaging system of fluorescence imaging technology.
Recently, the research team of Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences proposed a new near-infrared two-zone fluorescence mesoscopic system based on f-θ scanning scheme and confocal detection. This is the first near-infrared two-zone fluorescence mesoscopic imaging system. ， Breakthrough the limitations of NIR-II fluorescence imaging method can not achieve a large field of view, high resolution and excellent penetration depth while having the ability to optical slice.
This near-infrared two-zone fluorescence mesoscopic system uses a high-performance superconducting nanowire single-photon detector independently developed by Nanjing University, using special three-dimensional scanning and small hole filtering technology to achieve near-infrared two-zone mesoscopic imaging. In order to evaluate the performance of the system, the research team performed cerebral blood vessel imaging in mice, and the results showed that the imaging depth can reach 2.5 mm below the skull surface, and the lateral and axial resolutions were 6.3 µm and 160 µm, respectively, which can be clearly seen Microvessels, including main blood vessels and capillaries, can also provide depth analysis imaging function and zoom function. The experimental results show that the system is suitable for imaging large-area tissues, and can also obtain images with high spatial resolution, which provides new opportunities for exploring the mechanism of cerebrovascular diseases.
Fluorescence imaging technology is a common technology in basic research and clinical diagnosis and treatment of biomedicine, and it has been widely used in tumor diagnosis and treatment, detection of proteins and other substances in living organisms. The near-infrared two-zone fluorescence imaging technology has great potential for clinical transformation. The development of new imaging systems will expand the application of near-infrared two-zone fluorescence imaging technology. The next work will focus on the development of fluorescence lifetime imaging function and improvement of time resolution. The researchers hope that this can promote the further development of in vivo optical imaging.